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The Optimization of Plant Regeneration Protocol of Chinese Broccoli (Brassica oleracea L. var. alboglabra)
Huang Ke1,2, Yu Xiao Lin1, WU Qiu Yun2, Shen Ying chun1, Xiang Xun1, Cao Jia shu1*
1Institute of Vegetable Science, Zhejiang University, Hangzhou 310029, China; 2Institute of Vegetable Science, Fujian Academy of Agricultural Science, Fuzhou 350013, China
Abstract: By the orthogonalty design， the factors which influenced the regeneration of Brassica oleracea L. var. alboglabra were studied， the results showed that the major factor is the explant， and the next are NAA， BAP， sucrose and AgNO3， the best recipe of the culture medium for the regeneration is: MS+NAA 0.03 mg/L+BAP 2 mg/L+1% sucrose+AgNO37.0 mg/L+0.8% agar. The optimum explant is the hypocotyls with the regeneration frequency 97.5%.
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