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A Primary Study on the Water Osmotic Characteristics of Fibroblasts Employed in Tissue Engineered Dermal Replacem
WANG Xin, HUA Ze Zhao*, YANG Guang Hui1, CUI Lei1, LIU Wei1, CAO Yi Lin1, CHENG Qi Kang
Institute of Cryomedicine, Shanghai University of Science and Technology, Shanghai 200093, China; 1Shanghai Tissue Engineering Research & Development Center, Shanghai 200235, China
Abstract: TTo predict optimal protocols for cryopreservation of tissue engineered dermal equivalent， knowledge of fundamental cell water osmotic characteristics is required. In this study， the osmotic characteristics of cultured dermal fibroblasts were investigated using Coulter particle count and size analyzer. The normal cell volume is 5105.51mm3 (d = 21.40mm)measured at initial (isotonic) osmolality， 280.67 mOsm. The cell volume changes were measured after the cells were exposed and equilibrated to different anisosmotic conditions. The cell volume was found to be a linear function of the reciprocal of the extracellular osmolality (Boyle van''t Hoff plot) ranging from 130 to 1250 mOsm. The non-osmotic volume of the dermal fibroblast is 0.36 of the cells isotonic volume.
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