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The Effects of DON on HLA-I Expression of Human Peripheral Blood Mononuclear Cells in vitro
LI Yue Hong, ZHANG Xiang Hong, XING Ling Xiao, YAN Xia, WANG Jun Ling, WANG Feng Rong
Laboratory of Experimental Pathology, Hebei Medical University, Shijiazhuang 050017, China
Abstract: To explore the effects of deoxynivalenol on HLA-I expression of human peripheral blood mononuclear cells. Effects of deoxynivalenol at different concentration on HLA-I expression of human peripheral blood mononuclear cells in vitro were studied with flow cytometry(FCM) analysis and Western blotting. FCM analysis indicated that HLA-I expressions of human peripheral blood mononuclear cells were reduced in DON treatment groups (50ng/mL， 100ng/mL， 1000ng/mL and 2000ng/mL)， especially in relatively higher concentration DON (1000ng/mL and 2000ng/mL) groups (P<0.01)， compared to that in control. In the concentration range from 50ng/mL to 2000ng/mL， a significant concentration-depended negative correlation could be found between HLA-Ⅰexpression and DON concentration. Western blotting also confirmed that DON at higher concentration (1000ng/mL and 2000ng/mL) could significantly inhibit HLA-I expression at protein level. The results suggest that DON could reduce HLA-I expression of human peripheral blood mononuclear cells in concentration-dependent manner in vitro.
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