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Establishment and Biological Characteristic Analysis of a New ES Cell Line Derived from 129S1 Mouse Strain
YANG Hua, SUN Xia, XU Guo Jiang, LIU Wei, WANG Long, ZHAO Xu Dong, LU Rong Hua, FEI Jian, WANG Zhu Gang*
Shanghai Nan-Fang Research Center of Model Organisms, Shanghai 201203, China
Abstract: A new ES cell line was isolated and cultured from inner cell mass collected from 129S1 mouse embryos at early stage， named as NM-2. The biological characteristics of this cell line were observed. It shows the typical morphology of ES cell clone， express embryo stem cell specific markers: alkaline phosphatase and transcription factor OCT-4. The normal karyotype rate was 80%. NM-2 could differentiate into various tissues from all three primary germ layers in vivo. Following injection into blastocysts， 79% of newborn mice were chimeras and 31% of male chimera showed germ-line transmission. All mice derived from germ-line chimeras show normal phenotype. The results demonstrated that NM-2 cell line was a mouse ES cell line with high potential for germ-line contribution.
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