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A Modified Method for Cytoskeleton Staining of Muscle Cells
CHENG Yun Hui, HAN Mei*, WEN Jin Kun, ZHANG Yong Gang, LIU Zhi Min
Hebei Laboratory of Medical Biotechnology, Institute of Basic Medical Science, Hebei Medical University, Shijiazhuang 050017, China
Abstract: In this study， we improved classical staining method for cytoskeleton with coomassie brilliant blue R250 and compared it with immunofluorencence. The cultured vascular smooth muscle cells were transiently prefixed with paraformaldehyde， then stained with coomassie brilliant blue as routine procedures. The cytoskeleton stained by the modified method was clear and kept its nature shape. The paraformaldehyde prefixed-coomassie brilliant blue staining is a convenient cytoskeleton staining method for muscle cells.
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