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The Effect of Cryopreservation on the Acrosome of the Spermatozoas from SHR
CHEN Sun Hong*, WANG Ru Yao, LU Xin, QIN Shi Xiao, LI Jian Guo, WU Ming Zhang
Department of Histology and Embryology, Shanghai Second Medical University, Shanghai 200025, China
Abstract: With the best cryopreservation protocol and the cryoprotectant for rat spermatozoas we could found， the effect of cryopreservation on the acrosome of the spermatozoas from SHR were studied. The effect of cryopreservation on the acrosomal reaction of SHR spermatozoa is determined by the ways such as chlortetracycline (CTC) staining， sperm penetration of zona-free egg assay， and β-D-galactosidase activity analysis. The acrosomal reaction rate of frozen rat spermatozoas (13.4%) was significantly lower than that of unfrozen (68.6%). Compared to the spermatozoas unfrozen， the fertility index of frozen spermatozoas was significantly reduced (23.4±7.02 and 10.2±3.95， respectively); as well as that of β-D-galactosidase activity(9.39±1.98 and 4.50±1.40， respectively). The present results indicated that cryopreservation impaired the acrosome of rat spermatozoas.
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