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Inhibitive Effects of Antisense hTRT on Human Colonic Carcinoma Cells
FU Gui Lian, DONG Jun Hong*, DU Pei Ge
The Medical College of Beihua University, Jilin 132001, China
Abstract: hTRT was reversely inserted into pcDNA 3.0 and recombinant pcDRTRT was obtained. Recombinant pcDRTRT were transfected into colonic carcinoma cell line SW-111C were transfected into colonic carcinoma cell line SW-111C with lipofectin and a stable transfected cell line， ie. SW-111C-pcDRTRT was obtained. The transfected cells were charatered with easily dropping， slow growth， and loss of overlap growth ability; Flow cytometry(FCM) detection showed that the cell in G0/1 phases of SW-111C-pcDRTRT increased， but the cells in S and G2M phases decreased; The SW-111C-pcDRTRT cells couldn't form in soft agar; the hTRT's expression of SW-111C-pcDRTRT cells was significantly lower than that of control cells. The results indicated that telomerase activity of SW-111C cells transfected with antisense hTRT was decreased， cellular growth was inhibited and cellular malignant phenotype was reversed.
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