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Isolation and Culture of Porcine Preadipocyte In Vitro
Guo-Hua Zhang , Gong-She Yang, Chang-Qing Qu, Shi-Duo Sun*
Laboratory of Animal Fat Deposition and Muscle Development, Northwest Agriculture & Forestry University, Yangling 712100, China
Abstract: Explant techniques and monolayer culture in culturing porcine preadipocytes were compared， Cells obtained from monolayer culture are more than from explant techniques in a short period of time， but we have to purify cells by successive passage culture. Subcultured cells were plated in differentiateon medium， analyzed by their morphological changes， growth curve， oil red O staining and lipoprotein lipase gene， peroxisome proliferators-activated receptor g gene expression. Results shown a lot of preadipocytes could be harvested from adipose tissue samples obtained from crossbreed piglets， these preadipocytes were typical fibroblasts with normal morphology， proliferative and had some extent differentiation rate in primary culture. Under controlled condition， especially purified by subclutre， these preadipocytes replayed their hyperplasia process with higher accumulation of drop lipid .This study laid the basis for further probing into adipocyte proliferative， differentiation and fat deposition.
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