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Molecular Cloning and mRNA Expression Profiles of Leukocyte Cell-derived Chemotaxin 2 Gene (LECT2) in Ayu (Plecoglossus altivelis)
Hong-Yan Yang, Jiong Chen*, Yu-Hong Shi, Ming-Yun Li
Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, China
Abstract: Leukocyte cell-derived chemotaxin 2， also known as LECT2， is a protein related with multifunctional roles in cell growth， differentiation， damage/repair process and carcinogenesis to autoimmune diseases. A full-length cDNA clone of the LECT2 gene， 547 bp in size， 3'-end with a polyA tail， encoding a protein of 156 amino acids with a molecular weight of 17 kDa， was isolated from the fish ayu (Plecoglossus altivelis). Sequence comparison and phylogenetic analysis shows that ayu LECT2 (aLECT2) is most similar to rainbow trout LECT2， and the relationships of the different LECT2 coincided well with the evolutionary relationships of their organisms. The mRNA level of aLECT2 was highest in liver and moderately high in kidney and brain.
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