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Culture of Chicken Embryonic Primordial Germ Cells in Vitro
Bei Xie1,2, Hai Huang1,2, Xiao-Fen Hu2,4, Xiang-Rong Cao1, Qi-Zhong Huang2, Zhen Li2,3*
1 School of Life Science, Nanjing Normal University, Nanjin 210097, China; 2 Division of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of Agri-genetics and Breeding, Shanghai 201106, China; 3 Animal Husband
Abstract: Primordial germ cells (PGCs) were isolated from the blood of stage 14-15 chick embryos by using Ficoll density centrifugation. After isolated， primordial germ cells were cultured with or without stroma cells. The PGCs could survive 4 days when cultured in Medium 199 supplemented with FBS， chicken serum， bFGF， human IGF-1 and murine LIF， while they could be cultured for twenty-three generations when cultured on different growth factors and stromal cells derived from 5-day-old chicken germinal ridge. The PGCs could maintain a viability rate of 80% after being frozen and thawed.
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