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The Mechanism of Bone Marrow Mesenchymal Stem Cells Chemoattract Neural Progenitor Cells in Vitro
Qun Xue1,2, Zhong-Nin Miao1, Jun Hua3, Jing Qu1, Ming-Yuan Wang1,4, Jun Jin5, Qing Shi1, Yong-Jing Cheng1, Zheng-Yang Fang1,4, Guo-Zhen Hui5
1The Stem Cell Reaserch Laboratory of Jiangsu Province, Institute of Biology Technical, Soochow University, Suzhou 215006,China; 2 Department of Neurology, the First Affiliated Hospital of Soochow University, Suzhou 215006, China;
Abstract: Brain trauma examination indicated that both bone marrow mesenchymal stem cells (BMSC) and neural progenitor cells (NPC) could migrate after transplantation， in vivo， and integrated into the cells around of them， so as to accelerate the recovery of neurological impairment. Although the mechanism of it is not clear， it was speculated that some chemokines or cytokines secreted by BMSC might contribute to it. This study discussed the possible mechanism dealing with the molecular SDF-1α/CXCR4. BMSC were confirmed by ELISA to secret high level of SDF-1α [about (1860.00±526.29) pg/ml]. Suggested by Boyden Chamber Assay， BMSC were able to chemoattract the CXCR4 positive NPC migration in vitro， which could be neutralized by the monoclonal antibody CXCR4. It is speculated that TNF-α may up-regulate the expression of CXCR4 on the NPC， thus enhance the migration ability of NPC. This provided a novel idea to neurological impairment therapy by stem cells transplantation.
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