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Bone Marrow Mesenchymal Stem Cells Can be Induced to Differentiate into Cardiomyocytes-like in Vitro
Bo Wang, Gui-Ling Wang1, Xiao-Juan Bai*, Jin-Dan Song1
Department of Internal Circulation , No.1 Hospital Affiliated to China Medical University, Shenyang 110001, China; 1 Key Laboratory of Cell Biology of Ministry of Public Health of China, China Medical University, Shenyang 110001, China
Abstract: The bone marrow mesenchymal stem cells (BMMSCs) from wistar rats were cultured and expression of surface markers， including CD34， CD44， CDw90， were evaluated. After the first generation BMMSCs were treated with 10 µmol/L 5-azacytidine agent， they were checked by immunohistochemistry staining of antibodies against α-sarcomeric actin and troponin T in 2 and 4 week. We verified that the first generation BMMSCs could be induced to differentiate into cardiomyocytes-like. This provided theory foundation and technique method for cardiomyocytes induced in vitro and applied in clinic.
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