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Response of VEGF Expression in Human Brain Microvascular Endothelial Cell to the Inducer of Endoplasmic Reticulum Stress
Da-Peng Wang, Bo Li, Wen-Gang Fang, Yu-Hua Chen*, Jin-Dan Song
Department of Developmental Biology, Key Laboratory of Cell Biology of Ministry of Public Health of China, China Medical University, Shenyang 110001, China
Abstract: To reveal the expression of vascular endothelial growth factor (VEGF) under conditions that cause endoplasmic reticulum (ER) stress， human brain microvascular endothelial cells (HBMEC) were treated with tunicamycin at different concentration in vitro. RT-PCR， Western blot and immunocytochemisty analyses were used to measure intracellular levels of VEGF. The result showed that there was a basal expression of VEGF in HBMEC. Conditions known to active ER stress response could increase the expression of VEGF， and the level of VEGF mRNA was not correlated with that of VEGF under all conditions. Compared with GRP94， the increase of VEGF in response to tunicamycin at different concentrations was rapid. These results indicated that VEGF was an ER stress responsive gene and suggested that the expression of VEGF was regulated by unfolded protein response.
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