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Matrix Attachment Regions Increased Stable Integrated CAT Reporter Gene Expression Level in NIH3T3 Cells
Tian-Yun Wang, Fang Tian, Sheng-Li Zhang, Wei-Hong Hou, Jian-Min Wang, Le-Xun Xue*
Laboratory for Cell Biology, Zhengzhou University, Zhengzhou 450052, China
Abstract: The β-globin matrix attachment region (MAR) and β-interferon MAR DNA fragments were amplified through PCR， and cloned into the upstream of SV40 promoter of pCAT3 vector in forward and reverse orientation， the CAR enzyme activities of different constructs were examined under transient and stable expression respectively. The results showed that β-globin MAR could increase the CAT gene expression 8 fold， β-interferon MAR 3 fold in stable integration transformants， however there were little effects of MAR on CAT gene expression levels under transient expression. The orientation of two MARs inserted into the vector had no effect on CAT gene expression.
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