Home > Browse Issues > Vol.27 No.2
β-Amyloid Oligomers Regulate Munc-18 Expression in the Human Neuroblatoma SH-SY5Y Cells
Shu-Wen Qian, Qiong Feng, Cui-Qing Zhu*
State Key Laboratory of Medical Neurobiology, Shanghai Medical Center of Fudan University, Shanghai 200032, China
Abstract: This study is to explore whether the β-amyloid (Aβ)， a pathogenic factor of Alzheimer's disease， could affect Munc-18 expression. The assembly of monomer Aβ to oligomeric form was induced with Zn2+. The effect of oligomeric Aβ at low nanomolar concentration on Munc-18 expression of the differentiated human neuroblatoma SH-SY5Y cells was analyzed with western blot and flow cytometry methods. In addition， the cytotoxicity of oligomeric Aβ was also studied with MTT method. Results showed that Munc-18 protein expression significantly decreased at 1 day after 1 nmol/L Aβ oligomers treatment and followed by an increase at 2 day and 3 day. Similar changes of Munc-18 expression were also observed with higher Aβ concentration， but inhibitive effect on Munc-18 expression was more obvious. Meanwhile， oligomeric Aβ at low nanomolar concentration did not significantly inhibit metabolism of SH-SY5Y cells. We concluded that Aβ oligomers at low nanomolar concentration could regulate the expression of Munc-18 in differentiated neuroblastoma SH-SY5Y cells.
CN
EN