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The Protective Effects of Lycium bararum Polysaccharides on Alloxan-induced Rat Pancreatic Islets Damage
Hong-Feng Zhang*, Yu-Fei Wang1
Department of Biology, East China Normal University, Shanghai 200062, China; 1Diabetes Institute, Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China
Abstract: To study the protective effects of Lycium bararum polysaccharides (Lb-PS) on isolated pancreatic islets which were incubated with 4 mmol/L alloxan (AXN)， Insulin released into medium and in islets was detected by radioimmunoassay and glucokinase and SOD activity， NO and malonaldehyde (MDA) production were measured by colorimetric assay. Results indicated that AXN significantly inhibited the insulin release in response to glucose and insulin content in islets. In addition， there was a higher NO and MDA concentration and reduced glucokinase and SOD activity in islets incubated with AXN. Lb-PS could restore glucokinase and SOD activity， insulin synthesis and secretion inhibited by AXN， and reduce NO and MDA production in AXN-induced islets. Therefore Lb-PS could protect glucose-induced insulin synthesis and insulin secretion through reducing NO production and maintaining glucokinase and SOD activity in pancreatic β cells.
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