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Nuclear Transfer Using Zona-free Oocytes
Jun-He Hu, Chun-Rong Yang, Zhong-Ying Dou*
Shaanxi Province Branch Center of National Stem Cell Engineering & Technology Center, Northwest Sci-tech University of Agriculture and Forestry, Yangling 712100, China
Abstract: Although somatic nuclear transfer (NT) achieved a lot of progress in recent years， the ways of NT was still the same to the Willadsen's method: enucleation， injection， fusion and activation. Cloning animals showed many problems such as abnormally developing organs， but the reason was unclear. Therefore， it is necessary to innovate the ways of NT. A novel NT method that employing zona-free oocyte has been developed in order to improve the efficiency and solve exited problems of NT. This paper introduces two methods of zona-free NT: the reverse cloning and handmade cloning as well as the single embryo culture system.
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