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Serum-free Culture of Insect Cells
Cui-Ping Cao1, Xiao-Feng Wu1*, Xing-Meng Lu2
1Laboratory of Biological Resources and Gene Engineering, 2Laboratory of Invertebrate Pathology, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China
Abstract: Serum is commonly used as a supplement to cell culture medium. The most widely used animal serum is fetal bovine serum， FBS. With the improvement of modern cell biology in cell and tissue culture and the standardization of cell culture protocols， considerable ethical concerns were fixed recently on the harvest and collection of FBS. Thus， in order to decrease the annual need of animal serum in terms of the 3Rs， any reduction in the use or partial replacement of serum is expected. In addition， because of serum being an ill-defined component and in terms of an improvement of cell and tissue culture methodology， many serum-free cell culture medium were developed successfully and were well accepted as an alternative to the use of FBS in cell and tissue culture.
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