CircDOCK1 Affects Gastric Cancer Progression by Regulating the miR-128-3p/HMGB3 Axis

This article aims to explore the effect of circDOCK1 on gastric cancer cell functions and its mechanism. The expression levels of circDOCK1, miR-128-3p and HMGB3 in gastric cancer tissues, adjacent tissues, human gastric mucosal epithelial cells GES-1, and human gastric cancer cells HGC-27 were determined by qRT-PCR and Western blot. si-NC, si-circDOCK1, miR-NC, miR-128-3p mimic, si-circDOCK1+miR-NC inhibitor and si-circDOCK1+miR-128-3p inhibitor were transfected into HGC-27 cells, respectively. The HGC-27 cells were divided into si-NC group, si-circDOCK1 group, miR-NC group, miR-128-3p mimic group, si-circDOCK1+miRNC inhibitor group and si-circDOCK1+miR-128-3p inhibitor group. Targeting relationship of circDOCK1 and miR-128-3p was assessed by dual-luciferase reporter assay. CCK-8 method, clone formation experiment, scratch experiment, Transwell experiment and flow cytometry were used to detect cell functions. CircDOCK1 and HMGB3 levels were upregulated, while miR-128-3p level was downregulated in gastric cancer tissues and HGC-27 cells  (P<0.05). CircDOCK1 could target miR-128-3p to regulate HMGB3 expression. Transfection of si-circDOCK1 or miR-128-3p mimic promoted cell apoptosis (P<0.05), but inhibited cell proliferation, migration and invasion (P<0.05). In addition, miR-128-3p inhibitor reversed the promoting effect of si-circDOCK1 on HGC-27 cell apoptosis, as well as the inhibitory effect on cell proliferation, migration and invasion. CircDOCK1 promoted gastric cancer cell proliferation, migration, invasion, and induced apoptosis by miR-128-3p/HMGB3 axis, indicating that circDOCK1 might be a potential molecular target for the treatment of gastric cancer.

CSTR: 32200.14.cjcb.2024.12.0006