Research on the Endothelial Cell-Derived Matrix Induced by Lipopolysaccharide Regulates the Tight Junction Protein Expression in Human Hepatocytes
LI Weihong1, MA Yuejiao2, HE Xu1, WANG Ziyi2, GUO Xinyue2, ZHANG Haiyan2
This study was to analyze the dedifferentiation of human vascular ECs (endothelial cells) and the increased extracellular matrix secretion induced by LPS (lipopolysaccharide). The regulated effect and mechanism of the EC matrix (endothelial cell-derived extracellular matrix) induced by LPS on the expression of tight junction protein in human hepatocytes were study. The characteristics of endothelial cells, the components and ultrastructural changes of EC matrix were analyzed in human umbilical vein endothelial cells treated with control or LPS for 72 h. The changes of tight junction protein, integrin and DNA methylation and hydroxymethylation in human hepatocytes were analyzed after 72 h of culture on the control-EC matrix or LPS-EC matrix. LPS induced the dedifferentiation of human umbilical vein endothelial cells, and promoted the change of the composition and structure of extracellular matrix secreted by endothelial cells. LPS-EC matrix down-regulated the protein levels of Claudin 1 and integrin α5 proteins and DNA hydroxymethylation in human hepatocytes. This study indicated that the injury of vascular endothelial cell affected the tight junction protein in human hepatocytes.
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