Src Mediates the Adhesion and Migration of Vascular Smooth Muscle Cells Induced by Osteopontin

Abstract: To investigate the roles of tyrosine kinase Src and related signaling molecules in regulating the adhesion and migration of vascular smooth muscle cells (VSMCs)， the adhesion and migration of VSMCs were induced by osteoponin (OPN)， and the effect of Src specific inhibitor PP2 on VSMC adhesion and migration as well as focal adhesion kinase (FAK) and integrin-linked kinase (ILK) was studied. The results showed that the adhesion and migration of VSMCs induced by OPN were decreased to 76.6% and 33.8% of control (P<0.05)， respectively， after the cells were pre-treated with PP2 for 1 h. The level of phosphorylated FAK increased about 1.9 times after VSMCs were treated with OPN， compared with that of control. The immunoprecipitation and Western blotting showed OPN stimulation induced ILK dephosphorylation and inhibited the association of FAK with ILK， which was reduced to 46.4% of control. PP2 significantly inhibited the phosphorylation of FAK， antagonized ILK dephosphorylation induced by OPN and promoted the association of FAK with ILK. These results suggest that Src regulates the adhesion and migration of VSMCs through affecting the phosphorylation of FAK and dephosphorylation of ILK， and that Src may be involved in OPN-integrin-FAK signaling pathway.

CSTR: 32200.14.cjcb.2006.05.0024