Study on the Mechanism of circ_UBE2D2/miR-144-3p Regulating the Proliferation, Apoptosis, Migration and Invasion of Cervical Cancer SiHa Cells
HE Qiaochu*
The aim of this work is to explore the influence and its possible mechanism of circ_UBE2D2/ miR-144-3p molecular axis on the proliferation, apoptosis, migration and invasion of cervical cancer SiHa cells. si-NC, si-circ_UBE2D2, miR-NC, miR-144-3p mimics were transfected into SiHa cells. si-circ BE2D2 and antimiR-NC, or si-circ_UBE2D2 and anti-miR-144-3p were co-transfected into SiHa cells. The dual luciferase reporter experiment was used to detect the effect of miR-144-3p overexpression on the luciferase activity of wild-type vector WT-circ_UBE2D2 or mutant vector MUT-circ_UBE2D2. MTT assay, plate colony formation assay, flow cytometry, and Transwell assay were used to detect the proliferation, colony formation, apoptosis, migration and invasion of SiHa cells, respectively. Western blot was used to detect the protein expression of Cleaved-caspase3, Bax, and Bcl-2. Overexpression of miR-144-3p could reduce the luciferase activity of WT-circ_UBE2D2 (P<0.05) but not that of MUT-circ_UBE2D2. Compared with the si-NC or miR-NC group, the cell proliferation inhibition rate, apoptosis rate and the protein levels of Cleaved-caspase3, Bax were increased in the si-circ_UBE2D2 or miR-144-3p mimics group (P <0.05), while the number of cell clone formation, migration and invasion cells, and the protein level of Bcl-2 were decreased (P<0.05). Compared with the si-circ_UBE2D2+anti-miR-NC group, the cell proliferation inhibition rate, apoptosis rate and the protein levels of Cleaved-caspase3, Bax were reduced (P<0.05) in the si-circ_UBE2D2+anti-miR-144-3p group, while the number of cell clone formation, migration and invasion cells and Bcl-2 protein level were increased (P<0.05). circ BE2D2 interference could inhibit the proliferation, clone formation, migration and invasion, induce cell apoptosis of cervical cancer cells by up-regulating miR-144-3p.
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