Advances in High-Throughput Sequencing for the Study of Alternative Splicing
ZHANG Liya1, NIU Baohua1, SONG Ning2*
AS (alternative splicing) achieves the diversity of most proteins in eukaryotes by producing multiple different protein isoforms from a single gene, adding an additional regulatory layer for gene expression. Up to 95% of human genes can be spliced to produce multiple proteins with different functions. In addition, about 15% of human genetic diseases and cancers are associated with alternative splicing. The regulation of alternative splicing is a delicated set of interactive mechanisms that assist important biological processes such as cell development and differentiation. In recent years, development of high-throughput sequencing drives research on alternative splicing in the field of tissue-specific gene expression. However, it is still challenging to combine both technologies. The application of high-throughput sequencing in alternative splicing is summerized in this review, moreover this study analyze the problems existing in this technology for giving solutions, which provide new strategies and ideas to promote the development of this field.
CN
EN