Application and Prospect of Polyyne Rainbow Probes in Biological Research
LI Yujian1,2,3#, SHEN Weiwei1,2,3#, YIN Jinhuan1,2,3, ZHANG Xi1,2,3, CUI Yaning1,2,3, ZHAO Yuanyuan1,2,3, LIN Jinxing1,2,3*
Carbow (carbon rainbow) is a super-multiplexed Raman probe based on polyyne scaffolds, which can distinguish more than twenty kinds of rainbow probe molecules with different vibrational frequencies through SRS (stimulated Raman scattering) microscopy. Using the specific modification of probe molecules, Carbow can simultaneously image multiple organelles within living cells, as well as macromolecules such as proteins and lipids. The high photostability and low cytotoxicity of polyacetylene molecules combined with multi-channel optical detection of SRS will enable Carbow to have great application potential in the biological field. This article reviews the Raman labeling technology based on the SRS platform and the design synthesis principles and advantages of Carbow. Furthermore, the article summarizes the biological applications of Carbow for the specific labeling imaging of different organelles in living cells, cell and tissue immune-labeling, cell sorting and high-throughput analysis. Finally, the improvement of Raman labeling technology and its application in the field of plant science are prospected.
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