The Mechanism of 3-MA Enhancing Sensitivity of Nasopharyngeal Carcinoma Cells to Polo-Like Kinase Inhibitors

YUAN Yeqin^1,2, FU Yuting^1,2, YANG Wenjun^1,2, JIANG Binyuan^1,2*

( ¹The Department of Clinical Laboratory Affiliated Changsha Central Hospital, Hengyang Medical School, University of South China, Changsha 410004, China; ² Central Laboratory, Affiliated Changsha Central Hospital, Hengyang Medical School, University of South China, Changsha 410004, China)

Abstract:

The incidence of nasopharyngeal carcinoma is high in Southeast Asia and the southern provinces of China. Cell cycle chemotherapeutics are mostly used in the treatment of advanced nasopharyngeal carcinoma, and PLK1 (Polo-like kinase 1) inhibitors are a new type of cell cycle chemotherapeutics with certain application prospects. In this study, nasopharyngeal carcinoma cells 5-8F and 6-10B were used as models to preliminarily explore the application of Polo-like kinase inhibitors. Through Cell Counting Kit-8 experiment and flow cytometry, it was found that PLK1 inhibitors GW843682X or BI2536 combinate with PI3K inhibitor 3-MA significantly enhance the proliferation inhibition of nasopharyngeal carcinoma cells and induce more nasopharyngeal carcinoma cell death. Based on the detection results of cell cycle and apoptotic proteins, it is believed that 3-MA enhances the killing ability of Polo-like kinase inhibitors on nasopharyngeal carcinoma cells by promoting the activation of CDK1/Cyclin B1 complex. In conclusion, 3-MA can be used as a sensitizer for PLK1 inhibitory drugs to enhance its inhibitory effect on nasopharyngeal carcinoma cells. This study also provides theoretical references for clinical applications and basic research on related drugs.

CSTR: 32200.14.cjcb.2022.12.0006