In Vitro Study of Erythrocyte/Tumor Membrane Cloaked Nanoparticle as Biomimetic Nanocarriers for Highly Targeting Photodynamic Cancer Therapy
YAN Shaohui1, SUN Zhihong2, LIU Jie2, SUN Chengming1,2*
The hybrid cell membrane formed by erythrocyte membrane and 4T1 breast cancer cell membrane was cloaked on the surface of mPEG-PLGA loaded with Verteporfin to form a shell structure, and a novel biomimetic nano-delivery carrier RT@VNPs was synthesized. The basic properties of biomimetic nanocarriers were analyzed by transmission electron microscopy, Zetasizer Nano ZS instrument, ultraviolet-visible spectrometers, and fluorescence spectrometers, etc. On this basis, 4T1 cells were used to evaluate the cellular uptake, ROS generation, and killing abilities of RT@VNPs in vitro. In addition, the ability of RT@VNPs induced 4T1 cells to produce immunogenic cell death effects was further analyzed by measuring the immunogenic cell death-related indicators HMGB1 and ATP.
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