The Application of Histochemical Staining Technology in Experimental Course of Plant Anatomy and Plant Developmental Biology
TAN Chao, SONG Jia, WANG Xue, SUN Shuangli, MEN Shuzhen*
The development of angiosperm male gametophyte (pollen grain) involves a series of cellular activities, including mitosis, dynamic changes of vacuoles and germination of pollen tubes. Histochemical staining is a common method to study plant anther development and pollen fertility. In this paper, a corresponding experimental course is designed for the anatomical observation of reproductive organs in undergraduate botany course. In these experiments, the model plant Arabidopsis thaliana anthers at different developmental stages were stained with various reagents. The dynamic changes of nucleus, vacuoles and pollen tubes were observed by bright filed microscope or fluorescence microscope, and then the pollen fertility was determined. These experiments enable students to have a systematic understanding of the process of Arabidopsis thaliana anther development.
CN
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