Preparation of Immune Cells from Mouse Intestinal Lamina Propria and Establishment of Multi-Parameter Flow Cytometry Panel

Flow cytometry is a multi-parameter, high-throughput and rapid detection technique for single cells or biological particles based on scattered light signal and fluorescence signal, which has a wide application prospect in biomedical field. Flow cytometry is an important method for immunophenotypic and functional analysis, especially in the basic research of immunology. Among them, the preparation of high quality single cell suspension is the key step of flow cytometry analysis, which directly affects the results. The intestinal lamina propria of immune cells in mice is one kind of difficult to preparation of the original tissue sample. This study uses two kinds of enzyme digestion conditions and compares the cell yield, cell viability and immune cell markers, the results show that the collagenase VIII and DNase I enzyme solution method is more suitable for intestinal lamina propria of immune cells in mice. The establishment of this single cell preparation method can be used to more accurately analyze and quantify the population of intestinal immune cells by flow cytometry. The study provided a reference basis for revealing the influence of the sample preparation for experimental results, and a reliable method for the accurate analysis of immune cell populations in mouse intestinal lamina propria.

CSTR: 32200.14.cjcb.2022.06.0012