Cloning and Expression Analysis of LcSUN1 Gene from Luffa cylindrica

ZENG Meijuan¹, CHEN Mindong¹, LIU Jianting¹, YE Xinru¹, WANG Bin¹, LIN Jinhui², ZHU Haisheng¹*, WEN Qingfang¹*

(¹Fujian Key Laboratory of Vegetable Genetics and Breeding, Crops Research Institute, Fujian Academy of Agricultural Sciences, Fujian Engineering Research Center for Vegetables, Fuzhou 350013, China; ²Zhangzhou Agricultural Technology Extension Station, Zhangzhou 363000, China)

Abstract:

Fruit length is one of the important characters of Luffa cylindrica fruits. Previous studies have shown that SUN genes are related to fruit length, but there are few reports on SUN genes in Luffa cylindrica. In order to investigate SUN genes of Luffa cylindrica, four SUN genes were identified based BLAST on the genomic data of Luffa cylindrica, named LcSUN1-LcSUN4, further LcSUN1 was cloned by RT-PCR. Bioinformatics analysis showed that LcSUN1 contains a transmembrane domain and a SUN domain. Phylogenetic tree analysis showed that LcSUN1 was closely related to the SUN protein of Cucurbita moschata and Benincasa hispida. Real-time fluorescence quantitative PCR analysis showed that the expression of LcSUN1 was increased at the initial stage and then decreased during the fruit development of Luffa cylindrica. In addition, the expression level of LcSUN1 was significantly higher in short fruit than in long fruit of Luffa cylindrica twelve days after flowering. The results suggested that LcSUN1 might have conserved function in regulation of fruit length in Luffa cylindrica, which would provide a theoretical basis for the study of fruit length of Luffa cylindrica.

CSTR: 32200.14.cjcb.2022.06.0007