The Effect of lncRNA PSMA3-AS1 on the Proliferation, Migration and Invasion of Liver Cancer Hep3B Cells Through Targeted Regulation of miR-627-3p
SUN Qiyue1, TAN Guang2*
This study aimed to investigate the effects of lncRNA PSMA3-AS1 on the proliferation, migration and invasion of liver cancer cells and its possible mechanism. The expression levels of lncRNA PSMA3-AS1 and miR-627-3p in liver cancer tissues, paracancerous tissues and normal human liver epithelial cells THLE-3, human liver cancer cells MHCC97H, Hep3B and SK-HEP-1 were detected by qRT-PCR. si-NC, si-lncRNA PSMA3- AS1, miR-NC and miR-627-3p mimics were transfected into Hep3B cells, respectively. si-lncRNA PSMA3-AS1 and anti-miR-NC, as well as si-lncRNA PSMA3-AS1 and anti-miR-627-3p were co-transfected into Hep3B cells. The targeting relationship between lncRNA PSMA3-AS1 and miR-627-3p were detected by dual-luciferase reporter assay. Cell proliferation was detected by MTT assay, cell clonal formation was detected by plate colony formation assay, and cell migration and invasion were detected by Transwell assay. The expression levels of MMP2 and MMP9 proteins were detected by Western blot. The results of qRT-PCR assay showed that compared with paracancerous tissues, the expression of lncRNA PSMA3-AS1 in liver cancer tissues was increased (P<0.05), while the expression of miR-627-3p was decreased (P<0.05). Compared with THLE-3 cells, the expression of lncRNA PSMA3- AS1 in MHCC97H, Hep3B and SK-HEP-1 cells was increased (P<0.05), while the expression of miR-627-3p was decreased (P<0.05). The results of dual-luciferase reporter assay showed that lncRNA PSMA3-AS1 could target and bind miR-627-3p. The results of MTT assay, plate colony formation assay, Transwell assay and Western blot assay showed that the cell viability and the protein levels of MMP2 and MMP9 were decreased after transfection of si-lncRNA PSMA3-AS1 or miR-627-3p mimics (P<0.05), and the number of colony formation, migration and invasion cells was decreased (P<0.05). The results of MTT assay, plate colony formation assay, Transwell assay and Western blot assay showed that co-transfection of si-lncRNA PSMA3-AS1 and anti-miR-627-3p could restore the inhibitory effects of transfected si-lncRNA PSMA3-AS1 on Hep3B cell proliferation, clone formation, migration and invasion. Interfering the expression of lncRNA PSMA3-AS1 can attenuate the proliferation, clonogenesis, migration and invasion of liver cancer cells by targeting miR-627-3p expression.
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