miR-29 Family Inhibits the Proliferation, Migration and Invasion of Colon Cancer MC38 Cells Through the AKT/mTOR Signaling Pathway
ZHENG Cheng1,2, DONGFANG Yang2, CHENG Lei2, LIU Keke1,2, WU Wenjing2 *, ZHANG Jin2 *
Colon cancer is one of the most common malignant tumors. The number of deaths caused by colon cancer is second only to lung cancer. Studies have found that the expression of the miR-29 family members (miR-29a, miR-29b, and miR-29c) in colon cancer tissue was significantly lower than in paracancerous tissue, but its effect on colon cancer is unknown. In order to explore the effect and mechanism of the miR-29 family members on colon cancer, a colon cancer cell line MC38 was used for the transfection of miR-29a/b/c-3p mimics individually in this research. qRT-PCR, CCK-8 and EdU staining experiments proved that the miR-29 family members could inhibit the proliferation of MC38 cells (P<0.05). Cell wound scratch assay and Transwell invasion experiment showed that the miR-29 family members could suppress the migration and invasion of MC38 cells (P<0.05). Finally, the signaling pathway analysis and dual-luciferase report assay indicated that the miR-29 family members could decrease the activity of AKT/mTOR signaling pathway and interact with FSCN1 (Fascin actin-bundling protein 1). The above results indicated that the miR-29 family members could reduce the proliferation, migration and invasion of colon cancer cell line MC38 by inhibiting the activity of AKT/mTOR signaling pathway.
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