Effects of Residual Hydrogen Peroxide on the Proliferation and Biological Characteristics of MSCs and NK Cells in an Isolator after Decontamination

In this study, the effects of residual hydrogen peroxide disinfectant on the proliferation and biological characteristics of MSCs (mesenchymal stem cells) and NK (natural killer) cells were investigated. A low concentration of hydrogen peroxide disinfectant was used to establish a specific environment for MSCs and NK cell culture. The proliferation ability was analyzed by cell counting method, and cell surface markers were detected by flow cytometry. The differentiation potential of MSCs was tested by culturing under trilineage differentiation culture medium. The results showed that MSCs and NK cells had normal morohology and exhibited high proliferation ability in the environment of 3-5 mg/m³ concentration of hydrogen peroxide. The results of flow cytometry showed that CD73, CD105, and CD90 were highly expressed in MSCs, and CD45, CD34, CD14, CD79a and HLA-DR were negatively expressed; the percentage of NK cells expressing CD56+ and CD3– were more than 70%. After induction, MSCs exhibited multilineage differentiation capacity into adipocytes, osteoblasts and chondrocytes. In conclusion, MSCs and NK cells were growing well in low concentrations of hydrogen peroxide disinfectant environment, and there was no significant difference in proliferation and differentiation potential compared with the cells which cultured in normal cell culture environment.

CSTR: 32200.14.cjcb.2022.05.0001