Effect of Anticancer Bioactive Peptides on the Proliferation of Esophageal Cancer Cells Co-Cultured with Cancer-Associated Fibroblasts
LIANG Yabing1, YANG Ling1, ZHANG Man2, SU Xiulan1*
This work was to investigate the effect of CAFs (cancer-associated fibroblasts) on the proliferation of ESCC (esophageal squamous cell carcinoma) cells and the intervention effect of proliferation and possible mechanism of ACBP (anti-cancer biological active peptide). Five cases of esophageal cancer tissues were collected, from which ESCC CAFs were isolated and cultured, and then the characteristic indexes were identified; the co-culture system of CAFs and ESCC cells was established. CFSE staining and flow cytometry were used to detect cell proliferation; the conditioned medium of CAF-1 was collected, and ACBP was added to co-cultured KYSE140 cells; cell proliferation was detected by IncuCyte, and the expression levels of Hedgehog signaling pathway-related genes were detected by qRT-PCR and Western blot. CAFs were successful separated. The results of Western blot showed that CAFs expressed Vimentin and Fibronectin without E-cadherin. Compared with the control group, the CFSE level of KYSE140 cells decreased and the fusion rate increased (P<0.05), and the mRNA and protein levels of GLI1 and PTCH1 were increased (P<0.05) after co-cultured with CAF-1; compared with the conditional medium group, the fusion rate of KYSE140 cells decreased (P<0.05) after ACBP was added; compared with the control group, the mRNA and protein levels of GLI1 and PTCH1 of KYSE140 cells decreased after ACBP was added (P<0.05). This study suggests that CAFs can promote the proliferation of esophageal cancer cells by activating the Hedgehog signaling pathway, and ACBP can inhibit the growth of esophageal cancer cells by inhibiting the Hedgehog signaling pathway under the co-culture with CAFs.
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