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Home > Browse Issues > Vol.43 No.8

The Improvement Effect of Water-Soluble Fullerene C60 on Steatotic Hepatocytes


SU Qiang1, SUN Na1, CHENG Yan1, WANG Shuqin2, YU Meilan2, ZHANG Yong3*, CHEN Kan1,2*

(1The Key Laboratory of Biotechnology & Bioengineering (KLBB) of the State Ethnic Affairs Commission, Northwest Minzu University, Lanzhou 730030, China; 2College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China; 3The 940th Hospital of Joint Logistics Support of Chinese People’s Liberation Army Stationed in the First, Outpatient Department, Lanzhou 730050, China)
Abstract:

This study was to investigate the effect of water-soluble fullerene C60 on steatotic hepatocytes in vitro. Huh6 and WRL68 cell lines were induced by free fatty acid to establish steatotic hepatocytes models in vitro, and C60 was added to intervene the cells. Nile red staining was used to detect lipid accumulation. TG (triglyceride), ROS (reactive oxygen species), SOD (superoxide dismutase), GSH (glutathione) and ATP (adenosine triphosphate) were measured. The results showed that the accumulation of lipid droplets as well as the levels of TG and ROS were significantly increased in the cells induced by free fatty acids, but decreased in the cells treated with C60. In particular, for Huh6 cells which were sensitive to oxidative stress, C60 had better effect. In conclusion, C60 can effectively reverse the degree of steatosis and protect cell from injury induced by oxidative stress.


CSTR: 32200.14.cjcb.2021.08.0004