hESC-Derived Mesenchymal Stem Cells are Superior in Promotion of Angiogenesis in Vitro
YAN Yongli, YE Jinpei*
hESC (human embryonic stem cell) is a kind of pluripotent stem cells with unlimited proliferation ability and multi-lineage differentiation potential in vitro, which can be induced into a variety of cells including MSC (mesenchymal stem cell) under appropriate conditions. The present study systematically investigated the in vitro proangiogenic effect of hESC-MSC (hESC-derived mesenchymal stem cell), which was generated in this laboratory recently. The experiments included the effect of hESC-MSC’ conditioned culture supernatants on the function of HUVEC (umbilical vein endothelial cell) and their potential to differentiate into vascular endothelial cells, in comparison with human ADSC (adipose-derived mesenchymal stem cell) and UC-MSC (umbilical cord mesenchymal stem cell). It was found that the CM (conditioned medium) from hESC-MSC could significantly promote the proliferation and migration of HUVEC, and the proliferation of HUVEC was significantly enhanced with the CM from hESC-MSC compared with that from UC-MSC. Moreover, the differentiating hESC-MSC could form an extensive tube-like network in vitro after induction of differentiation towards vascular endothelial cells by all protocols tested, and the length of putative tubing was significantly longer than that of differentiating ADSC and UC-MSC, from which the tube-like structure formation appeared only occasionally. In conclusion, hESC-MSC can significantly promote angiogenesis in vitro, suggesting that this novel source of MSC may be able to become an ideal therapeutic cell type for cardiovascular diseases.
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