The Role of Osteoclast Extracellular Traps in the Pathogenesis of Rheumatoid Arthritis
ZHOU Hong, AN Yanan, NI Lihui, XU Hongyue, MA Fangxue, WANG Chao, LI Yuan, LIANG Junchao, YU Lu*
This paper mainly analyzes the mechanism of ETs (extracellular traps) released by OCs (osteoclasts). Immunofluorescence, scanning electron microscopy and immunoblotting techniques were used to investigate the the mechanism of ETs formation caused by treatment of osteoblasts with CII (type II collagen) and PMA (phorbolmyristate13-acetate), respectively. The results showed that both CII and PMA were able to induce the formation of OETs (osteoclast ETs). PMA induced the production of NOX-dependent ETs, while CII induced the production of NOX non-dependent ETs. The results also suggested that CII and PMA regulated the production of ETs through AKT activation. In conclusion, CII and PMA can induce the formation of ETs by osteoblasts. Investigating the mechanism of ETs production can lay the foundation for effective treatment of rheumatoid arthritis.
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