Interleukin-6 Down-Regulates eNOS Thr495/497 through AMPK/SIRT1 Pathway
LI Chang, YANG Xiyang, LUO Minghao, LUO Suxin*
This is a study on the effects of IL-6 (interleukin-6) on eNOS (endothelial nitric oxide synthase) of BAECs (bovine aortic endothelial cells) and its mechanism. BAECs were treated with IL-6 in different concentrations (10, 20, 40 ng/mL) and different times (0.5, 1, 2 h). Beside, BAECs were pre-treated with AMPKα1 siRNA and then were treated with IL-6 for 2 h (kept with AMPKα1 siRNA). The expression of eNOS, Thr497, AMPK (adenylate-activated protein kinase), p-AMPK, and SIRT1 (silence information regulator 2 related enzyme 1) in BAECs were detected by Western blot. The NO (nitric oxide) content in BAECs was measured by NO detection kit. The results showed that IL-6 significantly reduced the expression of Thr497 (P<0.05), accompanied by upregulation of p-AMPK, SIRT1 (P<0.05), and increased NO content compared with the normal control group. The down-regulation of Thr497 by IL-6 was blocked by AMPKα1 siRNA and EX-527 (P<0.05). In short, IL-6 could down-regulate the expression of Thr497 in BAECs and then affect eNOS activity. Its mechanism may involve the participation of AMPK/SIRT1 pathway.
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