Cloning and Expression Analysis of Lampetra japonica L-STAT3 in Vitro and in Vivo
ZHANG Xinyan1#, ZHANG Wenying1, 2#, LI Qingwei1, 2, 3, LI Yingying1, 2, 3*
As a member of the STAT (signal transduction and transcriptional activator) protein family, STAT3 plays important roles in many physiological processes, including cell differentiation and immunity. In this study, the full-length of STAT3 was cloned from Lampetra japonica and its amino acid sequence was homologous with those of other species, and then was named as L-STAT3. The prokaryotic expression and eukaryotic expression of L-STAT3 gene were performed respectively, while expression of L-STAT3 gene in E. coli was in the form of inclusion body. The nucleotide sequence of L-STAT3 was optimized according to the codon preference of Komagataella phaffii and the secretion expression of L-STAT3 could be achieved. The expression of L-STAT3 gene in different tissues of L. japonica was further examined at transcription and protein levels. These attempts provided technical support for obtaining important functional proteins of lamprey and laid a foundation for further verification of lamprey protein functions. In addition, the analysis of the expression pattern of L-STAT3 can provide a theoretical basis for studying the adaptive immune process of lamprey.
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