Abnormal Expression of Radil Affects Proliferation and Migration of Intrahepatic Cholangiocarcinoma Cells

Radil (Ras association and DIL domains) was an important factor that is previously known to regulate cell adhesion and cell migration. However, its functions have not yet been fully realized in various tumors. In this study, both characteristics of Radil expression and the Radil affected cell adhesion and cell migration were investigated in ICC (intrahepatic cholangiocarcinoma) tissues, in order to elucidate the molecular mechanism of regulation on Radil expressions. Assay of Real time-PCR and Western blot were used to detect the levels of mRNA and protein for Radil in collected ICC and peri-ICC tissues. Assays of CCK8, Transwell and wounding experiments were used to analyze its potential effects on cell proliferation and cell movement. Finally, assays of both Chip-PCR and Luciferase reporting system were used to determine the possible regulation of HIFs (hypoxia inducible factors) on Radil gene expression. Our results indicated that the expression levels of Radil in peri-ICC tissues were all significantly lower than those in ICC tissues. The increased Radil expression in ICC cells might promote their proliferation and migration levels, while reduced Radil expression in ICC cells decreased proliferation and migration levels. As a target gene, Radil was known to be regulated by HIFs. Together, our findings indicate that HIF-regulation promotes the high expression level of Radil, which further promotes both prolifera tion and migration of ICC cells. Our findings provide the potential application to use Radil as a marker for treating prognosis of ICC.

CSTR: 32200.14.cjcb.2020.09.0005