Function Study of TGFB Signaling Pathway on Islet β Cell Proliferation
LIU Tingsheng, ZHANG Fangfang, JIN Liang*
The aim of this study is to investigate the effect of TGFB (transforming growth factor β) signaling pathway on islet β cell proliferation. Firstly, TGFβ1, an activator of TGFΒ signaling pathway, and SB-431542, an inhibitor of TGFΒ signaling pathway, were used to activate and prohibit the TGFΒ signaling pathway in Min6 cells respectively. Then, CCK-8 was used to determine the cell viability of Min6 cells treated by TGFβ1 or SB-431542. FACS (fluorescence activated cell sorting) was applied to detect the proportion of Ki-67+ (Ki-67 positive) cells in each group after treatment of TGFβ1 or SB-431542 for 48 h. Finally, islets isolated from C57BL/6J mice were treated by TGFβ1 or SB-431542 for 48 h, and Insulin+&Ki-67+ cells in mice islets were detected by immunofluorescence. Results showed that cell viability of Min6 cells, the proportion of Ki-67+ Min6 cells and the amount of Insulin+&Ki-67+ cells of mouse islets were reduced by treatment of TGFβ1 compared with control groups. And cell viability of Min6 cells, the proportion of Ki-67+ Min6 cells and the amount of Insulin+&Ki-67+ cells of mice islets were upregulated by treatment of SB-431542 compared with control groups. These data reveal that inhibition of TGFB signaling pathway promotes islet β cell replication, which indicates a new therapy for diabetes.
CN
EN