Myostatin Regulates VEGFA Expression in MG-63 Cells Through the PI3K–Akt-c-Jun Signaling Pathway

ZHAO Jin¹, PAN Xinling¹, JIN Lulu¹, SU Zhenming²*

(¹Department of Biomedical Sciences Laboratory, Affiliated Dongyang Hospital of Wenzhou Medical University, Jinhua 322100, China; ²China Medical University, Taiwan 40402, China)

Abstract:

Myostatin and VEGFA highly expressed in patients with rheumatoid arthritis, but the relationship between them is unclear. In this study, the paper analyzed the expression of VEGFA by Western blot and RTqPCR. The phosphorylation levels of PI3K, Akt, c-Jun were detected after treatment with inhibitors or siRNAs. The results showed that VEGFA protein and mRNA expression increased when the cells were treated with myostatin, and the highest level was at the concentration of 3 ng/mL. After myostatin treatment, the phosphorylation degrees of PI3K, Akt and c-Jun protein increased, and the up-regulation effect of myostatin on VEGFA protein was weakened after the corresponding inhibitor treatment. Myostatin up-regulates the expression of VEGFA in MG-63 cells through the PI3K-Akt-c-Jun signaling pathway.

CSTR: 32200.14.cjcb.2020.07.0004