Home > Browse Issues > Vol.42 No.5

Study on Morphological Characteristics and Embryonic Development of the Gynogenesis Silurus lanzhouensis

LI Lanlan^1,2, XIAO Wei^2,3,4, XING Lumei^1,2, SAI Qingyun^2,3,4, LIU Yanbin^2,3,4, TIAN Yonghua^2,3,4, WANG Yan^2,3,4, YU Zhaoxi^2,3,4, LIU Zhe¹*, LIAN Zongqiang^2,3,4*

(¹College of Animal Science and technology, Gansu Agricultural University, Lanzhou 730070, China; ² Ningxia Fisheries Research Institute, Yinchuan 750001, China; ³Ningxia Fishery Engineering Technology Research Center, Yinchuan 750001, China; ⁴Academician Workstation of Ningxia Fishery Science and Technology, Yinchuan 750001, China)

Abstract:

In this study, the sperm of Pelteobagrus fulvidraco was UV-irradiated and used to fertilize eggs of Silurus lanzhouensis as well as diploid gynogenesis (G2n) was induced using cold shock. The diploid gynogenesis (G2n), haploid (N), hybrid diploid (H2n), and homodiploid embryo (2N) were compared in development timing and biological characteristics of embryo development. The results were as follows: (1)Fertilization rates were 2N>H2n>N>G2n, Abnormality rates were 2N>G2n>N, Hatching rates were N>G2n>2N. (2)At the temperature of 23 °C, the ordinary diploid embryo hatched out in 48 h, gynogenesis diploid hatched out in 51 h, hybrid diploid ceased to develop in

neurula stage (26 h 20 min), and the haploid ceased to develop in pre-hatching stage. (3)The differences in the developmental morphology of the embryos in each group mainly manifested in the fact that the hybrid diploid did not form a normal embryo body, but the haploid showed a typical haploid syndrome. Compared with the ordinary diploid, newly hatched larvae of gynogenetic diploid was no significantly different in morphology. The study provides a method for the gynogenetic technique of S. lanzhouensis, as well as a theoretical basis for cytological studies on the development of haploid, hybridized, and gynogenetic embryos of this species.

CSTR: 32200.14.cjcb.2020.05.0009