Single-Cell Sequencing Technology and Its Research Progress in Plant cells

HE Qizouhong^1,2, E Yilan^1,2, LI Ruili^1,2*

(¹Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing 100083, China; ²National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China)

Abstract:

Traditional sequencing technology requires bulk sample to provide an average view of gene expression and ignore the information of gene expression heterogeneity between individual cells. Single-cell sequencing, as an emerging technology, provides new ideas for solving the above problems. Currently, this technique has been wildly used in animal cell studies, but fewly used in plants cell. This review mainly focuses on the development history and key technologies of single-cell sequencing technology, with special emphasis on the research progress in plant cells, which will provide valuable information for further study on plant single-cell genome and transcriptome sequencing.

CSTR: 32200.14.cjcb.2020.04.0020