The Effect and Mechanism of miR-24-3p on Proliferation and Migration in Human Cervical Cancer Cells
HUANG Yiyun1, HU Lijun1, LIN Lu1, PENG Qi1, HU Qin1, ZHA He2, ZHOU Lan1*
This study aimed to investigate the role of miR-24-3p in the proliferation and migration of cervical cancer cells and its mechanism. miR-24-3p inhibitors were used to down-regulate the expression of miR-24- 3p in cervical cancer cells, then MTT Transwell assays and Western blot were used to measure cell proliferation, migration and the protein level of the proliferating cell nuclear antigen. The target genes of miR-24-3p were predicted and then used for function annotation through bioinformatic methods. The relationship between miR-24- 3p and target gene AMOTL2 (angiomotin-like 2) was verified by dual-luciferase reporter system and Western blot, siRNA inhibited AMOTL2 was used to detect its effect on cervical cancer cell migration. Data showed that downregulating miR-24-3p could inhibit the proliferation and migration ability of cervical cancer cells and reduce PCNA protein expression. Its target genes were mainly present in the cell-cell junction component, and were significantly enriched in biological processes including protein kinase activity molecular function, proteins autophosphorylation and microRNA in cancer signaling pathway. miR-24-3p was a negative regulator of the optimal target gene AMOTL2, and the decreased expression of AMOTL2 promoted the migration of cervical cancer cell CaSki. In conclusion, miR-24-3p can regulate multiple target genes, which are involved in varied biological processes and signaling pathways, also promote cell proliferation and facilitate cell migration by targeting AMOTL2 in cervical cancer.
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