Preliminary Study on the Use of Dictyostelium discoideum in Comprehensive Undergraduate Laboratory Course of Cell Biology— Transformation of Lifeact-Green Fluorescent Protein by Electroporation
GAO Runchi, WU Xue, NI Juan, ZHOU Tao, WANG Xiaoyan*
Dictyostelium discoideum is a powerful model organism that is ideal for studying cytokinesis, cell movement, phagocytosis, chemotaxis, electrotaxis, signal transduction, and cell differentiation. This experiment describes the use of electroporation technology to transform the Lifeact-GFP (Lifeact-green fluorescent protein) into living cells of Dictyostelium discoideum, then screening for positive clones with resistance to hygromycin B. The Lifeact-GFP-labeled microfilaments distributed in Dictyostelium discoideum are observed by fluorescence microscopy. Through comprehensive laboratory course training, the interest of learning and the ability of applying to theoretical knowledge of undergraduate students can be improved, thereby training the students in scientific thinking.
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