Effects of Maslinic Acid on H2O2-Induced Oxidative Injury and Pathway of p38 and Nrf2/HO-1 in BRL-3A Cells
LI Guilan*, ZHENG Kaifeng, QIN Shaogang, CHEN Lei, YUE Caixia, GUO Yongqi
In the paper, the protective effect and the mechanism of maslinic acid on oxidative injury of BRL-3A cells induced by H2O2 were investigated. Cell viability was determined by MTT. The intracellular ROS levels were measured using DCFH-DA. The activities of LDH, SOD and CAT as well as the contents of GSH and MDA were assayed by micro-enzyme-linked method. The mRNA levels of Nrf2, HO-1 and p38 were investigated by reverse transcription polymerase chain reaction. Western blot was used to evaluate the protein levels of Nrf2, HO-1 and p-p38. The results showed that maslinic acid attenuated the cell viability loss, the intracellular ROS levels and LDH leakage induced by H2O2. The activities of SOD and CAT, the GSH content were increased, but the level of MDA was reduced by maslinic acid. In addition, the nuclear translocation of Nrf2 could be promoted. The expression of HO-1 both in mRNA level and protein level was up-regulated. The phosphorylation of p38 was inhibited by maslinic acid. These results suggested that maslinic acid protected against H2O2-induced oxidative injury in BRL-3A cells, and its mechanism was probably associated with the promotion of Nrf2 nuclear translocation, the up-regulation of HO-1 expression and the inhibition of p38 phosphorylation.
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