The Relationship Between the Radioresistance of Hypopharyngeal Carcinoma Cells FaduRR and Epithelial Mesenchymal Transformation
WANG Haiyan1,2, WANG Zhihai1, HU Guohua1*
The aim of this study was to explore the relationship between the radioresistance FaduRR cell and the EMT (epithelial-mesenchymal transformation) as well as its potential mechanism in hypopharyngeal squamous carcinoma. The radioresistant hypopharyngeal cancer cell FaduRR was previously established with a gradient cumulative irradiation dose. The radioresistance of FaduRR was measured by colony forming assay and CCK8 cell viability kits. The apoptosis rate was measured by flow cytometry. The migration and invasion abilities were investigated by wound healing assay and transwell assay. Moreover, the mRNA and protein levels were detected by Western blot and qRT-PCR assay. Compared with Fadu cells, FaduRR cells radiation sensitivity was significantly lower. The CCK8 cell viability kits results showed that the survival rate of FaduRR cells in different doses on the fourth day after irradiation were higher than that of parental cells, the apoptosis rate of FaduRR cells was lower. The radioresistant cells FaduRR demonstrated typical ‘cobblestone’ or spindle-like shape, with pseudopodia and enlarged intercellular space. The invasiveness and migration abilities of the FaduRR cells were enhanced. The mRNA and protein expression levels of E-cadherin were significantly decreased, while the mRNA and protein expression levels of N-cadherin and Vimentin were markedly increased after as compared with parental cells. In the Akt/GSK-3β/Snail signaling pathway, the protein levels of p-Akt, p-GSK-3β and Snail were significantly increased, while the expression of Akt and GSK-3β was not significantly changed. According to the results, the radioresistant hypopharyngeal cancer cell FaduRR can induce the epithelial mesenchymal transformation, and the specific mechanism may be related to the activation of Akt/GSK-3β/Snail signaling pathway.
CN
EN