Expression of circCapzb in Endometrium during Peri-Implantation Period of Early Pregnancy Mice

CircRNA (circular RNA), as a new class of endogenous non-coding RNAs, though closely related to the occurrence and progression of various diseases, is rarely investigated during embryo implantation. The purpose of this study was to investigate the expression of circCapzb in peri-implantation period endometrium of pregnant mice. The expression levels of circCapzb in embryo tissues in IS (implantation sites) and IIS (inter implantation sites) were detected by Real-time PCR from day 5 (d5) to day 7 (d7) of pregnancy. In vivo model of induced decidualization in mice and in vitro model of induced decidualization in primary mice endometrial stromal cells were constructed respectively, and the expression of circCapzb in the models was detected by Real-time PCR. The circCapzb downstream targets miRNAs were miR-377-3p and miR-7005-5p. They were predicted by bioinformatics, and their expression in the decidual induction model was detected by Real-time PCR. The results showed that circCapzb expression was significantly higher at the implantation sites from day 5 to day 7 than at the interimplantation sites. CircCapzb expression in tissue and cell decidualization induction in vivo and in vitro model was significantly higher in the induced group than in the non-induced group. The expression of miR-377-3p and miR-7005- 5p downstream targets of circCapzb in the tissue and cell decidualization induction in vivo and in vitro model was significantly lower in the induced group than in the non-induced group. This study showed that circCapzb was highly expressed in mice embryo implantation sites during early pregnancy, and in tissue and cell decidualization in vivo and in vitro induction models. circCapzb might play a role in mouse endometrial decidualizationduring early pregnancy, but the specific mechanism remained to be further studied.

CSTR: 32200.14.cjcb.2020.03.0004