Research Progress in Extracellular Vesicles Detection and Analysis
ZHANG Wei1, YU Li2, CHEN Yang3*
Intercellular communication by dynamic extracellular vesicular compartment is a process conserved in both prokaryotes and eukaryotes. Compelling evidence supports the significance of extracellular vesicles that carry proteins, lipids or nucleic acid, in a broad range of physiological and pathological processes. Extracellular vesicles are identified in various body fluids, including blood, urine, saliva, breast milk, amniotic fluid, cerebrospinal fluid and bile, which make this compartment great candidates for biomarkers in disease diagnosis. Here in this review, we addressed the molecular contents and functions of extracellular vesicles in various body fluids. We also reviewed the standard methods for clinical sample collection, storage, handling and quality control for purification of extracellular vesicle to ensure the reliable sources for research.
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