Mechanism of Mouse Bone Marrow Mesenchymal Stem Cells Regulating Epithelial Sodium Channel via miR-130b
ZHANG Honglei, ZHOU Zhiyu, HOU Yapeng, LIU Hongfei, WANG Lin, DING Yan, NIE Hongguang*
The aim of this study was to investigate the effects of mouse BMSCs (bone marrow mesenchymal stem cells) on ENaC (epithelial sodium channel) via miR-130b. The isolated and cultured mouse BMSCs were seeded onto the transwell inserts and then co-cultured with H441 cells. The effect of BMSCs on the viability of H441 cells was detected by CCK-8 reagent. The effect of BMSCs on the level of γ-ENaC protein in co-cultured H441 cells was detected by Western blot. The expression of miR-130b in H441 cells co-cultured with BMSCs was detected by qRT-PCR. The microRNA was then transfected into normal cultured H441 cells, and its effect on γ-ENaC in H441 cells was further verified at the protein level. The results showed that BMSCs could enhance the viability of H441 cells. BMSCs could increase the protein level of γ-ENaC and the transcription level of miR-130b in cocultured H441 cells respectively. Western blot analysis further confirmed that miR-130b could increase the protein expression of γ-ENaC after transfection into H441 cells. Thus, we speculate that BMSCs can enhance the viability of H441 cells and may exert its regulation of γ-ENaC protein level through miR-130b.
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